V-730 UV-Visible

V-730 UV-Visible Spectrophotometer

 

The V-730 UV-Visible is a spectrophotometer manufactured by Jasco, a company known for providing instruments for scientific analysis. The V-730 is a specific model in their line of UV-Visible spectrophotometers, designed to measure the absorption of ultraviolet and visible light by a sample, typically in the range of 190 nm to 1100 nm.

Features:

1. Wavelength Range: The V-730 covers a wide range of wavelengths, typically from 190 nm to 1100 nm, which allows it to analyze both UV and visible light absorption.
2. High-Performance Optics: The spectrophotometer features high-quality optical components that improve the precision and accuracy of measurements.
3. User-Friendly Interface: The V-730 comes with an easy-to-use touchscreen interface, making operation intuitive for both beginners and experienced users.
4. Double Beam Technology: This system enhances accuracy by comparing the sample and reference beam, compensating for any fluctuations in light intensity over time.
5. Data Storage and Connectivity: The V-730 allows for the storage of measurement data and results, and it typically has USB or other ports for easy data transfer to a computer or external device.
6. Variety of Accessories: It is compatible with a range of accessories like microcells, fiber optics, and temperature controllers, allowing for more specialized measurements.
7. Applications: The V-730 can be used in various industries, including pharmaceuticals, environmental analysis, food and beverage, and chemical industries. It’s useful for tasks such as determining concentration of substances, kinetic studies, quality control, and more.

Applications:

• Concentration Measurements: Determining the concentration of a substance in solution by measuring absorbance at specific wavelengths using the Beer-Lambert law.
• Quality Control: Monitoring the consistency of products or raw materials in industries such as food, pharmaceuticals, and chemicals.
• Kinetics Studies: Monitoring the change in absorbance over time for reactions, making it useful for enzyme assays, reaction rate studies, etc.
• Environmental Monitoring: Measuring contaminants in water and air, including pollutants or trace metals.
• Protein and Nucleic Acid Analysis: Analyzing UV absorption spectra to quantify proteins, DNA, and RNA samples in research and clinical labs.

1. High Accuracy and Precision

• The double beam optical system in the V-730 ensures greater accuracy in measurements by compensating for fluctuations in light source intensity. This provides precise and reliable data, making it ideal for quantitative analyses, such as determining concentration levels of a substance.

2. Wide Wavelength Range (190-1100 nm)

• The spectrophotometer covers both the UV (ultraviolet) and visible regions, allowing users to measure a broad spectrum of substances. This versatility enables applications across various industries (e.g., pharmaceuticals, environmental monitoring, food testing, etc.) and for a wide range of samples (proteins, DNA, chemicals, etc.).

3. Ease of Use

• The V-730 is designed with a user-friendly touchscreen interface that simplifies operation. Users can quickly access features, set up methods, and view results with minimal training. The intuitive interface reduces the learning curve, making it accessible to both novice and experienced users.

4. Fast and Efficient Measurements

• The instrument allows for rapid measurements, providing fast results without compromising accuracy. This is especially important in high-throughput environments where time is a critical factor (e.g., pharmaceutical quality control, clinical diagnostics).

5. Versatility and Customization

• With a wide range of available accessories (e.g., temperature controllers, microcells, fiber optics), the V-730 can be customized for specific applications. This flexibility makes it suitable for both routine and advanced analytical tasks.

6. Data Storage and Connectivity

• The V-730 has the ability to store measurement data, which is particularly beneficial for ongoing projects, audits, or regulatory compliance. It also supports data export (e.g., via USB) to computers or external devices for further analysis and documentation.

7. Reliable Performance in Diverse Applications

• Whether used for routine quality control or more specialized research applications, the V-730 is highly adaptable. It’s commonly used in fields such as:
  • Pharmaceuticals: For drug analysis and quality control.
  • Environmental monitoring: For measuring pollutants in water, air, or soil.
  • Biotechnology: For protein, nucleic acid, and enzyme activity studies.
  • Food and Beverage: For ingredient analysis, color measurement, or nutritional content.
  • Chemicals: For concentration determination or chemical reactions monitoring.

8. Cost-Effective

• Compared to higher-end, research-grade instruments, the V-730 offers a good balance of performance and cost. It’s suitable for laboratories with moderate budgets but still requires high-quality analytical tools.

9. Compliance with Standards

• The V-730 often meets or exceeds regulatory and industry standards (e.g., FDA and USP), which is essential for industries like pharmaceuticals or food testing that need to adhere to strict quality and safety guidelines.

10. Support for Kinetic Studies

• The ability to monitor absorbance changes over time enables users to perform kinetic studies of reactions or processes. This feature is valuable in enzyme assays, reaction rate analysis, and other dynamic studies.

11. Compact and Durable Design

• The V-730 is built with durability in mind, offering long-lasting performance. Its compact design also allows it to fit in a variety of lab setups, saving space without sacrificing functionality.

12. Environmental Impact

• Some models of the V-730 are designed with energy efficiency in mind, reducing the environmental footprint of their use, which is a growing concern in laboratory operations.

V-730 UV-Visible Spectrophotometer:

Optical System

• Optical Configuration: Double beam
• Detector: Photodiode array (PDA)
• Light Source: Deuterium lamp (UV) and Tungsten halogen lamp (Visible)
• Wavelength Range: 190 nm to 1100 nm
• Wavelength Accuracy: ± 0.3 nm
• Wavelength Resolution: 1 nm (bandwidth)
• Wavelength Precision: ± 0.1 nm
• Photometric Range: -3.0 to +3.0 Abs (absorbance mode)
• Photometric Accuracy: ± 0.002 Abs at 1.0 Abs (at 500 nm)
• Photometric Precision: ± 0.001 Abs at 1.0 Abs (at 500 nm)
• Stray Light:
  • UV: ≤ 0.03% at 220 nm (NaNO2)
  • Visible: ≤ 0.03% at 340 nm (K2Cr2O7)

Performance

• Linearity: ± 0.002 Abs (at 0 to 1.0 Abs)
• Signal to Noise Ratio: > 2 × 10⁴ (at 500 nm)
• Stability: ≤ 0.002 Abs/h (at 500 nm)
• Scanning Speed: 200 nm/min (default) (up to 1200 nm/min)
• Data Interval: 0.1 nm to 5 nm (depending on scan range)

Sample Handling

• Sample Compartment: 3-position sample holder (standard)
• Cell Holder: Universal for 10 mm, 1 cm path length cells (standard), optional holders for 1 mm, 5 mm, or micro cells
• Path Length Range: 0.1 mm to 100 mm (depending on accessories)
• Volume Requirement: As low as 0.5 mL (with micro cells)

User Interface

• Display: 7-inch color touchscreen display
• Software: JASCO’s UV/Vis Spectrophotometer software for data collection, analysis, and reporting
• Connectivity: USB, Ethernet (for remote control and data transfer)
• Data Output: USB memory stick, LAN, printer

Measurement Modes

• Absorbance: Measure sample absorbance at specific wavelengths.
• Transmittance: Measure how much light passes through a sample.
• Concentration: Using a calibration curve, determine the concentration of an analyte.
• Kinetics: Measure the rate of change of absorbance over time.
• Photometric: Measure the absorbance at multiple wavelengths across a spectrum.
• Scan Mode: Full-spectrum scans across a range of wavelengths.

Applications & Features

• Spectral Scans: Full-spectrum scanning for qualitative and quantitative analysis.
• Kinetic Studies: Monitor changes in absorbance over time for reaction monitoring.
• Quantitative Analysis: Using the Beer-Lambert Law for concentration calculations.
• Data Storage: Stores measurement data and results for later access.
• Multiple Accessory Compatibility: Includes support for a range of accessories like temperature controllers, fiber optic probes, and more.

Environmental Conditions

• Operating Temperature: 10°C to 35°C (50°F to 95°F)
• Relative Humidity: 30% to 85% (non-condensing)

Power Requirements

• Power Supply: 100–240 V AC, 50/60 Hz
• Power Consumption: Approximately 200 W (depending on configuration)

Dimensions & Weight

• Dimensions (W × D × H): Approx. 450 mm × 360 mm × 200 mm (17.7 in × 14.2 in × 7.9 in)
• Weight: Approx. 10 kg (22 lbs)

Optional Accessories

• Temperature Controller: For temperature-dependent experiments.
• Micro Cell Holder: For small-volume samples (down to 0.5 mL).
• Fiber Optic Probes: For remote or in-line measurements.
• Automatic Sample Changer: For high-throughput analysis.

Optical System	Rowland off-circle arrangement Single monochromator Double beam type
Light source	Halogen lamp, Deuterium lamp
Wavelength range	190 to 1100 nm
Wavelength accuracy	+/-0.2 nm (at 656.1 nm)
Wavelength repeatability	+/-0.1 nm
Spectral bandwidth (SBW)	1 nm
Photometric range	-3~3 Abs
Photometric accuracy	+/-0.0015 Abs (0 to 0.5 Abs) +/-0.0025 Abs (0.5 to 1 Abs) +/-0.3 %T Tested with NIST SRM 930D
Photometric repeatability	+/-0.0005 Abs (0 to 0.5 Abs) +/-0.0005 Abs (0.5 to 1 Abs) Tested with NIST SRM 930D
Scanning speed	10-8000 nm/min
Slew speed	24,000 nm/min
RMS noise	0.00004 Abs (0 Abs, wavelength: 500 nm, measurement time: 60 sec, SBW: 1 nm)
Baseline stability	0.0004 Abs/hour (Value obtained more than one hour after turning on the source, when the room temperature is stabilized, wavelength: 250 nm, response: slow)
Baseline flatness	+/-0.0005 Abs (200 – 1000 nm)
Detector	Silicon photodiode
Standard functions	IQ accessories, Start button, Analog output
Standard programs	Abs/%T meter, Quantitative analysis, Spectrum measurement, Time course measurement, Fixed wavelength measurement, Validation, Daily maintenance, Two wavelength time course measurement
Dimensions and weight	486(W) x 441(D) x 216(H) mm, 15 kg
Power requirements	120 VA
Installation requirements	Room temperature: 15-30 Celsius, humidity: below 85%